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KMID : 0359320120520010025
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Korean Journal of Veterinary Research
2012 Volume.52 No. 1 p.25 ~ p.31
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Diagnosis of canine brucellosis using recombinant ribosomal protein L7/L12
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Lee Hyang-Keun
Kim Jong-Wan
Ha Yun-Mi
Her Moon
Kim Ji-Yeon
Lee Ki-Chan
Kang Sung-Il
Jung Suk-Chan
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Abstract
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Brucella (B.) canis is mainly transmitted by direct or indirect contact with aborted fetuses and placenta. It's also known to be able to infect human, which likely results in providing veterinarians and companion animal owners for infectious risk. To develop diagnostic ELISA, we cloned and expressed rp1L gene of B. canis, which encodes the ribosomal protein L7/L12. Using this purified recombinant protein, indirect-ELISA (iELISA) was evaluated using 78 positive and 44 negative sera. The sensitivity and the specificity of iELISA were 94% and 89%, respectively. The results indicated that indirect-ELISA using recombinant ribosomal protein L7/L12 may be useful for diagnosis of canine brucellosis.
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KEYWORD
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Brucella canis, indirect-ELISA, ribosomal protein L7/L12
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